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. 2016 Mar 30;7:11056. doi: 10.1038/ncomms11056

Figure 6. RA treatment potentiates meiosis-related changes in Max expression-ablated ESCs.

Figure 6

(a) Representative images of meiosis-like SYCP3-staining patterns in Dox-treated Max-null ESCs cultured with or without RA in the presence or absence of LIF for 6 days. (b) Expression levels of the Stra8 gene in Dox-treated Max-null ESCs cultured under the indicated conditions for 6 days. The Stra8 gene expression level in Dox-untreated control Max-null ESCs cultured in the presence of LIF was arbitrarily set to one (n=3, means±s.d.). (c) Frequency of meiosis-like SYCP3-staining patterns in Max-null ESCs treated with Dox for 6 days or together with RA in the presence or absence of LIF. (d) Effect of RA on the recovery of viable Dox-treated Max-null ESCs cultured with or without LIF. The number of cells at day 0 was arbitrarily set to one (n=3, means±s.d.). (e) Quantification of Stra8 mRNA levels in Dox-treated or -untreated Max-null ESCs cultured in the presence or absence of the RA receptor inhibitor AGN for the indicated days (n=3, means±s.d.). (f) Effect of the expression of Cyp26b1 on Stra8 expression levels in Max-null ESCs. (g) Dose-dependent effect of RA on Stra8 expression levels in Max-null ESCs cultured in N2B27 with no RA as basal medium (n=3, means±s.d.).