Fig. 1.

Phenotype of CV-MSCs and flow cell sorting. a Surface markers of CV-MSCs were evaluated by FACS analysis. CV-MSCs positively expressed CD105, CD73, CD166, CD29, CD90, HLA-ABC, CD54, and VCAM-1, and hardly expressed CD14, CD45, CD31, CD144, CD133 and HLA-DR. A representative sample is shown. b VCAM-1⁺CV-MSCs and VACM-1⁻CV-MSCs were separated by the BD Aria III cell sorting system. c Morphology of VCAM-1⁺CV-MSCs and VCAM-1⁻CV-MSCs (scale bar = 200 μm). CV chorionic villi, MSC mesenchymal stem cell, SSC side scatter, VCAM-1 vascular cell adhesion molecule 1