Fig. 5.

Human cytokine antibody array displayed the angiogenic secretome of VCAM-1⁺CV-MSCs. a Expression of 120 cytokines in SN of VCAM-1⁺CV-MSCs and VCAM-1⁻CV-MSCs was determined by human cytokine antibody array (AAH-CYT-G1000). VCAM-1⁺CV-MSC and VCAM-1⁻CV-MSC SN derived from two healthy donors were used. Each sample was performed in duplicate. The differential angiogenic cytokines between VCAM-1⁺CV-MSCs and VCAM-1^–CV-MSCs were similar in two healthy donors. b Cytokine signal >200 was analyzed, and ratio of cytokine signal in VCAM-1⁺CV-MSCs to VCAM-1⁻CV-MSCs was calculated. This was statistically significant if the cytokine signal ratio was >1.3 or <0.75. Data revealed that VCAM-1⁺CV-MSCs secreted more abundant angiogenic cytokines than VCAM-1^–CV-MSCs, including HGF, IL-8, ANG, ANGPT2, μPAR, CXCL1, IL-1β, IL-1α, CSF2, CSF3, MCP-3, CTACK, and OPG. CV chorionic villi, MSC mesenchymal stem cell, VCAM-1 vascular cell adhesion molecule 1. See Abbreviations for cytokine definitions