Fig. 2.

Gating strategy for the determination of dendritic cells and macrophages in the spleen. First living cells were selected based on size in the forward side scatter plot. Next MHC2⁺ and F4/80⁺ cells were selected as macrophages. Within the remaining MHC2⁺ and F4/80⁻ cells, myeloid DCs were gated as CD11c⁺CD11b⁺ and lymphoid DCs as CD11c⁺CD11b⁻. In both subsets, the expression of CD80 and CD103 was measured. All isotype controls were set at 1 %