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. 2016 Mar 30;7:11084. doi: 10.1038/ncomms11084

Figure 1. RingoA KO males are sterile.

Figure 1

(a) RingoA-deficient mice are indistinguishable from their littermates. (b) RingoA immunoprecipitates from WT and KO testis lysates were immunoblotted for RingoA. The asterisk indicates IgGs. (c) RingoA KO testes are about four times smaller than the WT testes in 2-month-old mice. (n=9 WT and 5 KO, P value=0.001). (d) Testis from 2-month WT and KO mice were fixed in Bouin's solution, embedded in paraffin and sections (6 μm) were stained with H&E. A, A-type spermatogonia; In, intermediate spermatogonia; Sc, spermatocytes; Se, Sertoli cell; Sg, spermatogonia in anaphase. Scale bar, 50 μm. (e) Paraffin sections from WT and KO testes were stained with TUNEL (green) and DAPI (blue). Scale bar, 10 μm. TUNEL+ spermatocytes per seminiferous tubule were counted. (n=48 WT and 114 KO tubules, P value=0.009). Error bars are presented as the average with s.d. Asterisks indicate statistical significance (**P value<0.01) determined by the unpaired two-way Wilcoxon rank-sum test. The uncropped immunoblot is shown in Supplementary Fig. 7.