Table 1.

Mixed leukocyte reaction (MLR)

Conditions	MUTZ-3 DC medium	Contact Co-culture
	medium	Control Fibro	IPF Fibro
Lymphocyte Proliferation (mean cpm ± SD, n = 3)	56,870 ± 6110	40,236 ± 6047	34,322 ± 2843
p value (versus MUTZ-3 DC)		0.02	0.01
Conditions	MUTZ-3 DC medium	Transwell Co-culture
	medium	Control Fibro	IPF Fibro
Lymphocyte Proliferation (mean cpm ± SD, n = 3)	36,967 ± 4942	29,328 ± 3215	26,890 ± 6564
p value (versus MUTZ-3 DC)		0.04	0.05

MLR was performed by culturing with 100 000 lymphocytes (depleted of adherent mononuclear cells by a 2 h-lasting culture) and 20 000 MUTZ-3 DCs previously cultured alone (medium) or co-cultured with control or IPF fibroblasts for 48 h (as in Fig. 1) in 200 μl RPMI complete medium (n = 6 for each condition) for 6 days During the last 18 h, 1 μCi of 3H-thymidine (Perkin-Elmer Wallac) was added per well. Incorporated radioactivity was determined using a MicroBeta plate reader (Wallac WS-Trilux 1450

Results are presented as mean (±SD) lymphocyte proliferation induced by MUTZ-3 DCs previously alone (medium) or co-cultured with control or IPF fibroblasts either in direct contact or in transwell conditions during 48 h before MLR

3H-thymidine incorporation of 105 lymphocytes alone was 605 ± 436 cpm and 3H-thymidine incorporation of 20 000 MUTZ3 DC alone was 138 ± 59 cpm, (mean ± SD, n = 6)