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. 2016 Apr 5;16:59. doi: 10.1186/s12866-016-0674-y

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© Bartosik et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Milestones in construction of unstable BHR vector. Circular maps of intermediate (a, b and c) and final vectors (d, e and f) are drawn to scale. Only intact orfs are indicated. Unique or double restriction sites important for cloning are shown, those described in the text are in bold. T₁ marks the divergent transcription terminator sequence T_pro/T_lyz of P1 prophage [49]