Table 2.
Oligonucleotides used in this study
| No | Name | Sequence |
|---|---|---|
| 1 | klcApRA3L | GCGCATGCGGGAGCGTGATCGTTACGGT |
| 2 | klcApRA3R | GCGGATCCATTGCAGCCATACGGCGAGG |
| 3 | klcAsmaF | GGCCCGGGTGCTCGTCTCGTCGGTCTG |
| 4 | xylEPscR | GGACATGTCATCTGCACAATCTCTGCA |
| 5 | KBRK2Apa | CCGAATTCGGGCCCGAAGATGGAGATTTCCCAATGACTGC |
| 6 | KBRK2Sal | CCGTCGACCGCTGTCTTTGGGGATCAGCCCTC |
| 7 | lacOMCS1 | CATGGAATTGTGAGCGCTCACAATTTCAGATCTGAATTCTACGTACTCGAGCTCGGAATTGTGAGCGCTCACAATTTCA |
| 8 | lacOMCS2 | CGCGTGAAATTGTGAGCGCTCACAATTCCGAGCTCGAGTACGTAGAATTCAGATCTGAAATTGTGAGCGCTCACAATTC |
| 9 | parSbg2a | GATCGGTTGCTTGTTCCACGTGGAACAAGGCCG |
| 10 | parSbg2b | GATCCGGCCTTGTTCCACGTGGAACAAGCAACC |
| 11 | NrulacIF | GGTCGCGACTGAATCCGGTGAGAATGG |
| 12 | PvulacIR | CGCGATCGATAAGCTTGCAATTCGCG |
| 13 | oriTRK2Fs | ACGGTCGACACATGTCTGGTTGGCTTGGTTTCATC |
| 14 | oriTRK2Rs | CGGAATTCACATGTTTGCCAAAGGGTTCGTGTAG |
| 15 | oriTRA3F | CGCGTCGACACATGTTTTAGCACAAGCGGCGGCAG |
| 16 | oriTRA3R | CGGAATTCACATGTAGTTAGGGGAAGCCGACGAG |
| 17 | Nco | ACCATGGTCATG |
| Primers used in site-directed mutagenesis | ||
| 18 | muttrfA RK2 | GTCCTTGAGAAAGGAGACgtc gGTTTAGCTA |
| 19 | muttrfA RK2 | CCAATGTTTAGCTAAACc gacGTCTCCTTTC |
| 20 | xhoF8180 | CGGGCCGTCGGCTCGaGCATCATATCGAC |
| 21 | xhoR8180 | CGATATGATGCtCGAGCCGACGGCCCGC |
| 22 | apaF9950 | CTTTCTGGTTGGCCGgGcCCAAAGTTTTtATCGTTTGGTTTCC |
| 23 | apaR9950 | GAAACCAAACGATaAAAACTTTGGgCcCGGCCAACCAGAAAGGC |
| 24 | Ppu618F | CGCTGCAGAGGCCAGACCCCGTGAAATT |
| 25 | Ppu618R | GCAAGCTTGGTCAGCATAGTCCACCTCA |
| Primers used for Real Time qPCR | ||
| 26 | galKF | ATGATCTTTCTTGCCGAGCG |
| 27 | galKR | AGCAGCTTTATCATCTGCCGC |
| 28 | trfAF | GTGAAGATCACCTACACCGGC |
| 29 | trfAR | TGGCAAAGCTCGTAGAACGTG |
| 30 | repBRA3F | CATCGAGAAGCAAAAGGCG |
| 31 | repBRA3R | CCAACTTGCGTAGGTCTTCCAG |
Restriction enzyme recognition sites are in bold, mutated nucleotides are indicated by lowercase, parS palindrome is underlined