Figure 5.

Effects of NM on macrophage subpopulation gene expression. Macrophages, isolated 3 and 7 days after exposure of rats to PBS CTL or NM, were incubated with anti–rat-FcRII/III antibody, and then stained with antibodies to CD11b and CD43 as described in the Figure 4 legend. Viable cells (DAPI⁻) were sorted into CD11b⁻CD43⁻, CD11b⁺CD43⁺, and CD11b⁺CD43⁻ subpopulations and processed immediately for RT-PCR analysis of expression of pro- (CCR2, iNOS, IL-12α) and anti- (IL-10, CX₃CR1, ApoE) inflammatory genes. Data were normalized to glyceraldehyde 3-phosphate dehydrogenase and presented as fold change relative to resident CD11b⁻CD43⁻ macrophages from CTL rats. Error bars, mean ± SE (n = 3–4 rats). *Significantly different (P ≤ 0.05) from CD11b⁺CD43⁺ macrophages. ^#Significantly different (P ≤ 0.05) from CD11b⁻CD43⁻ macrophages.