Table 1.
Expression of CD43 and CD68 by Lung Macrophages
| Macrophages | Phenotype | Cells (× 106) |
|
|---|---|---|---|
| CTL | NM | ||
| Resident (CD11b−) | CD43− | 10.8 ± 0.9 | 5.2 ± 1.2* |
| CD43+ | ND | ND | |
| CD68− | 0.5 ± 0.1 | 1.0 ± 0.1* | |
| CD68+ | 9.9 ± 0.1 | 3.0 ± 0.6* | |
| Infiltrating (CD11b+) | CD43− | 0.2 ± 0.1 | 2.1 ± 0.4* |
| CD43+ | 0.1 ± 0.1 | 2.7 ± 0.6* | |
| CD68− | ND | 1.0 ± 0.4* | |
| CD68+ | 0.1 ± 0.01 | 2.5 ± 0.5* | |
Definition of abbreviations: AF, AlexaFluor; CTL, control; ND, not detectable; NM, nitrogen mustard.
Cells, isolated 3 days after exposure of rats to PBS CTL or NM, were incubated with AF488 anti-CD11b and AF647 anti-CD43, or AF647 anti-CD68 antibodies. Number of resident (CD11b−) and infiltrating (CD11b+) macrophages expressing CD43 and CD68 were then calculated by flow cytometry based on forward and side scatter followed by doublet discrimination of live cells, relative to the total number of lung cells recovered. Data are presented as mean ± SE (n = 3–4 rats).
*
Significantly different (P ≤ 0.05) from CTL rats.