Figure 1.

Characterization of primary human nonsmoker and smoker airway basal cells. Immunohistochemical characterization of cytopreps of primary human airway basal cells isolated using selective culture methods from large airway epithelial samples obtained by bronchoscopy from healthy nonsmokers and healthy smokers. Shown are cytokeratin 5 (KRT5; basal cell), tetraspanin-24 (CD151; basal cell), secretoglobin family 1A member 1 (SCGB1A1; secretory cell), mucin 5AC (MUC5AC; secretory cell), β-tubulin IV (ciliated cell), and chromogranin A (CHGA; neuroendocrine cell). Scale bar, 20 μm. Data shown are representative images from a single primary donor sample obtained for each phenotype.