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. 2016 Feb;54(2):241–249. doi: 10.1165/rcmb.2015-0079OC

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Figure 2. — Effect of methylated oligonucleotides on gene expression and promoter methylation of PDE4D in nonasthmatic and asthmatic ASMCs. (A) mRNA expression level of PDE4D and (B) CpG site–specific methylation (%) of PDE4D (+366 to +592) assayed by MSPCR in nonasthmatic (five donors) and asthmatic (five donors) ASMCs transfected with PDE4D-targeting (M-PDE4D) or nontargeting methylated oligonucleotide control (M-CTL). β-actin was used as the reference gene to normalize the amount of bisulfite-treated DNA template. Methylated DNA standards (0, 25, 50, 75, and 100% methylated DNA) were used when determining percentage of DNA methylation of samples from qPCR. *P < 0.05 in comparison to lipofectamine (LF) control of nonasthmatic ASMCs; ^#P < 0.05 in comparison to M-CTL of asthmatic ASMCs.