Table 2.
Overview of sponge isolates screened for quorum quenching activity (QQ) and antibacterial activity.
| Isolates | Similarity | Closest related | Accession number | Class | Genera | QQ assay | Antimicrobial activity | ||
|---|---|---|---|---|---|---|---|---|---|
| CV026 | PAO1 | BS | EC | ||||||
| Ac10 | 100 | Pseudovibrio denitrificans UST4-50 | KM196102 | Alphaproteobacteria | Pseudovibrio | − | − | − | − |
| Ac11 | 99 | Bacillus flexus SBANSCa10 | KR186190 | Bacilli | Bacillus | − | − | − | − |
| Ac12 | 98 | Labrenzia sp. FGH455 | JQ342690 | Alphaproteobacteria | Labrenzia | − | − | + | − |
| Ac13 | 100 | Alphaproteobacterium 5PC-32 | EF657795 | Alphaproteobacteria | − | − | + | − | |
| Ac14 | 99 | Alphaproteobacterium GUDS1146 | KF282463 | Alphaproteobacteria | + | − | − | − | |
| Ac15 | 99 | Alphaproteobacterium 5PC-32 | EF657795 | Alphaproteobacteria | + | − | + | − | |
| Ac16 | 100 | Bacterium 2D802 | JF411487 | Alphaproteobacteria | − | − | − | − | |
| Ac17 | 96 | Pseudovibrio japonicus U1021 | AB734990 | Alphaproteobacteria | Pseudovibrio | + | − | − | − |
| Ac18 | 97 | Bacterium 1H203 | JF411464 | Alphaproteobacteria | − | − | − | − | |
| Ac2 | 97 | Pseudovibrio japonicus M4B33 | LN812991 | Alphaproteobacteria | Pseudovibrio | − | − | − | − |
| Ac3 | 100 | Bacillus flexus P9 | KR935237 | Bacilli | Bacillus | − | − | − | − |
| Ac4 | 100 | Alteromonadales bacterium GUDS1275 | KF282544 | Gammaproteobacteria | Alteromonas | + | − | − | − |
| Cc26 | 99 | Halobacillus virgiliensis S22 | AM161502 | Bacilli | Halobacillus | − | − | − | − |
| Cc27 | 99 | Nautella sp. ZJ2706 | KP301105 | Alphaproteobacteria | Nautella | + | − | + | − |
| Cc28 | 100 | Bacillus aerius rawirorabr 15 | LC092833 | Bacilli | Bacillus | − | − | − | − |
| Cc29 | 100 | Bacillus subtilis ASC4 | GU227615 | Bacilli | Bacillus | − | − | − | − |
| Cc30 | 98 | Oceanobacillus sp. Ma-21 | KF958190 | Bacilli | Oceanobacillus | − | − | − | − |
| Cc31 | 98 | Oceanobacillus sp. Ma-21 | KF958190 | Bacilli | Oceanobacillus | − | − | − | − |
| Cc32 | 98 | Oceanobacillus sp. Ma-21 | KF958190 | Bacilli | Oceanobacillus | − | − | − | − |
| Cc33 | 100 | Bacillus endophyticus HSL6 | KP866218 | Bacilli | Bacillus | − | − | − | − |
| Cc34 | 100 | Bacillus endophyticus HSL6 | KP866218 | Bacilli | Bacillus | − | − | − | − |
| Cc35 | 100 | Bacillus subtilis Pls8 | KR709146 | Bacilli | Bacillus | − | − | − | − |
| Cc36 | 99 | Gracilibacillus sp. IBP-VN3 | HM587330 | Bacilli | Gracilibacillus | + | − | + | − |
| De102 | 100 | Alpha proteobacterium 9PC-7 | EF657826 | Alphaproteobacteria | − | − | − | − | |
| De103 | 100 | Vibrio fortis | KM041184 | Gammaproteobacteria | Vibrio | − | − | − | − |
| De104 | 100 | Vibrio fortis CAIM 1769 | HM584115 | Gammaproteobacteria | Vibrio | + | − | + | − |
| De105 | 100 | Vibrio pelagius | KM041188 | Gammaproteobacteria | Vibrio | − | − | − | − |
| De106 | 100 | Vibrio fortis M1C_12m_09 | KM041184 | Gammaproteobacteria | Vibrio | − | − | − | − |
| De107 | 97 | Hahellaceae bacterium Ez249 | HE818298 | Gammaproteobacteria | Hahellaceae | − | − | − | − |
| De108 | 99 | Vibrio sp. S2-26 | KM216237 | Gammaproteobacteria | Vibrio | − | − | − | − |
| Pv100 | 99 | Altererythrobacter marinus H32 | NR_116432 | Alphaproteobacteria | Altererythrobacter | − | − | − | − |
| Pv101 | 100 | Alteromonas sp. PRIM-21 | KJ210054 | Gammaproteobacteria | Alteromonas | − | − | + | − |
| Pv79 | 99 | Bacterium RB26DO | KP684372 | Actinobacteria | − | − | − | − | |
| Pv81 | 99 | Bacillus sp. NCIM2637 | KT291161 | Bacilli | Bacillus | − | − | − | − |
| Pv82 | 98 | Erythrobacter sp. HME6855 | HQ449708 | Alphaproteobacteria | Erythrobacter | − | − | − | − |
| Pv83 | 98 | Alcanivorax sp. E46 | KJ364611 | Gammaproteobacteria | Alcanivorax | − | − | − | − |
| Pv85 | 100 | Micrococcus luteus CL10 | KR780393 | Actinobacteria | Micrococcus | − | − | − | − |
| Pv86 | 100 | Erythrobacter sp. CUA-870 | KJ732928 | Alphaproteobacteria | Erythrobacter | + | − | − | − |
| Pv87 | 94 | Formosa sp. CZ-BI2 | JX306765 | Flavobacteriia | Mangrovimonas | + | − | + | − |
| Pv88 | 99 | Marine bacterium SE96 | AY038924 | Gammaproteobacteria | Halomonadaceae | + | − | + | − |
| Pv90 | 97 | Bacillus nitritophilus IB-256 | AJ309562 | Bacilli | Bacillus | + | − | − | − |
| Pv91 | 100 | Dietzia maris IHBB 9296 | KR085794 | Actinobacteria | Dietzia | + | − | + | − |
| Pv93 | 99 | Alcanivorax sp. JXH-324 | KR012312 | Gammaproteobacteria | Alcanivorax | − | − | + | − |
| Pv95 | 100 | Mycobacterium sp. 13-17-1 | KM886164 | Actinobacteria | Mycobacterium | − | − | + | − |
| Pv96 | 98 | Erythrobacter sp. 32NM FL03 | KM357374 | Alphaproteobacteria | Erythrobacter | − | − | − | − |
| Pv97 | 98 | Alcanivorax sp. 2A75 | AB435642 | Gammaproteobacteria | Alcanivorax | − | − | − | − |
| Pv98 | 100 | Staphylococcus pasteuri SMJ33 | KT036409 | Bacilli | Staphylococcus | + | − | − | − |
| Pv99 | 99 | Alcanivorax sp. 2A17 | AB435641 | Gammaproteobacteria | Alcanivorax | − | − | − | − |
| Sc19 | 99 | Rhodococcus nanhaiensis SCSIO 10197 | JN582176 | Actinobacteria | Rhodococcus | − | − | + | − |
| Sc22 | 95 | Alphaproteobacterium D21 | DQ399723 | Alphaproteobacteria | − | − | − | − | |
| Sc23 | 96 | Vibrio ponticus H078 | KJ577073 | Gammaproteobacteria | Vibrio | − | − | − | − |
| Ss111 | 99 | Kocuria sp. MNmon19 SNSum1 | KP639593 | Actinobacteria | Kocuria | − | − | − | − |
| Ss113 | 99 | Streptomyces sp. 1_C7_48 | EF540458 | Actinobacteria | Streptomyces | − | − | − | − |
| Ss114 | 99 | Halomonas sp. JSM 102013 | KR109186 | Gammaproteobacteria | Halomonas | − | − | − | − |
| Ss115 | 99 | Idiomarina sp. JL974 | DQ985042 | Gammaproteobacteria | Idiomarina | − | − | − | − |
| Ss116 | 99 | Halomonas alimentaria HQB612 | KT758546 | Gammaproteobacteria | Halomonas | − | − | − | − |
| Ss37 | 100 | Vibrio tubiashii 04/002-1T2 | KF270470 | Gammaproteobacteria | Vibrio | − | − | − | − |
| Ss38 | 99 | Marinobacter sp. LV10R510-8 | KF384121 | Gammaproteobacteria | Marinobacter | + | − | − | − |
| Ss39 | 99 | Vibrio owensii HQB622 | KT758552 | Gammaproteobacteria | Vibrio | − | − | − | − |
| Ss42 | 100 | Vibrio sp. BL0118 | KT731385 | Gammaproteobacteria | Vibrio | − | − | − | − |
| Ss43 | 99 | Vibrio harveyi isolate M1B39 | LN812999 | Gammaproteobacteria | Vibrio | − | − | − | − |
| Ss44 | 99 | Erythrobacter vulgaris Nordv6 | KC462903 | Alphaproteobacteria | Erythrobacter | − | − | − | − |
| Ss45 | 99 | Halomonas sp. JSM 102013 | KR109186 | Gammaproteobacteria | Halomonas | − | − | − | − |
| Ss5 | 100 | Kocuria rosea 0112ALTP5 | LN867187 | Actinobacteria | Kocuria | − | − | − | − |
| Ss52 | 100 | Alcanivorax sp. JXH-324 | KR012312 | Gammaproteobacteria | Alcanivorax | − | − | − | − |
| Ss53 | 97 | Roseibium denhamense M2B5 | LN812987 | Alphaproteobacteria | Roseibium | − | − | − | − |
| Ss54 | 99 | Chromohalobacter sp. Sa11 | AB305300 | Gammaproteobacteria | Chromohalobacter | − | − | − | − |
| Ss56 | 100 | Sinomicrobium oceani AAl04, | KJ754140 | Flavobacteriia | Sinomicrobium | − | − | − | |
| Ss58 | 100 | Vibrio harveyi ATCC 33843 | CP009467 | Gammaproteobacteria | Vibrio | − | − | − | − |
| Ss6 | 99 | Planococcus rifietoensis CCMM B654 | FR695449 | Bacilli | Planococcus | − | − | + | − |
| Ss60 | 99 | Alcanivorax sp. NCCP-902 | AB970645 | Gammaproteobacteria | Alcanivorax | − | − | − | − |
| Ss61 | 100 | Halomonas shengliensis RB 40 | KJ939459 | Gammaproteobacteria | Halomonas | − | − | − | − |
| Ss62 | 99 | Bacterium SCSIO13137 | KJ802238 | Gammaproteobacteria | − | − | − | − | |
| Ss63 | 100 | Paracoccus sp. PaH2.06a | GQ391948 | Alphaproteobacteria | Paracoccus | + | − | − | − |
| Ss64 | 100 | Micrococcus sp. SK57 | LC068964 | Actinobacteria | Micrococcus | − | − | − | − |
| Ss66 | 99 | Planomicrobium okeanokoites NA-7 | KC967059 | Bacilli | Planomicrobium | − | − | − | |
| Ss68 | 100 | Paracoccus sp. JSM 2185010 | KR109058 | Alphaproteobacteria | Paracoccus | + | − | − | − |
| Ss69 | 100 | Micrococcus sp. SK57 | LC068964 | Actinobacteria | Micrococcus | − | − | − | − |
| Ss7 | 99 | Kocuria polaris XJB-YJ7-2 | KM186611 | Actinobacteria | Kocuria | + | − | − | − |
| Ss70 | 99 | Bacterium RH70DO | KP684403 | Actinobacteria | − | − | − | − | |
| Ss71 | 99 | Bacillus sp. 9RB4 | KT150223 | Bacilli | Bacillus | − | − | − | − |
| Ss72 | 100 | Staphylococcus cohnii RCB1038 | KT261250 | Bacilli | Staphylococcus | − | − | − | − |
| Ss73 | 100 | Vibrio sp. NB0080 | KP770094 | Gammaproteobacteria | Vibrio | − | − | − | − |
| Ss74 | 99 | Bacterium RH70DO | KP684403 | Actinobacteria | − | − | − | − | |
| Ss76 | 99 | Vibrio sp. PB43 | KT731397 | Gammaproteobacteria | Vibrio | − | − | − | − |
| Ss77 | 99 | Vibrio harveyi NS131631 | KR003734 | Gammaproteobacteria | Vibrio | − | − | + | − |
Strain ID provides information on the sponge source of each isolate (Ac, Amphimedon chloros; Cc, Crella cyathophora; De, Diacarnus erythraenus; Pv, Pione vastifica; Ss, Sarcotragus sp. and Sc, Suberites clavatus). Closest 16S rRNA sequence based on % identity, and accession numbers were retrieved from NCBI database using BLASTn, and did not include uncultured/environmental sample sequences. Abbreviations used for isolates indicate the source sponge species: (Ac), Amphimedon chloros; (Cc), Crella cyathophora; (De), Diacarnus erythraenus; (Pv), Pione vastifica; (Ss), Sarcotragus sp. and (Sc), Suberites clavatus.