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. Author manuscript; available in PMC: 2016 Apr 5.
Published in final edited form as: Mol Cell Biochem. 2015 Sep 8;410(1-2):187–195. doi: 10.1007/s11010-015-2551-7

Fig. 2.

Fig. 2

27-OHC reduces p53 activation. a Luciferase reporter assay in MCF7 cells demonstrates a decrease in p53 activity in the presence of 0.1 μM 27-OHC, 1 μM 27-OHC, or 2 nM Estradiol (E2) for 24 h. b No change in p53 activity was detected with the luciferase reporter assay in the ER-negative MDA-MB 231 cells treated with 0.1 μM or 1 μM 27-OHC for 24 h. c Luciferase reporter assay in MCF7 cells demonstrates that fulvestrant, an ER inhibitor, attenuates 27-OHC-induced p53 inactivity when treated with 1 μM 27-OHC and/or 5 μM of fulvestrant. Data are expressed as Mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 versus vehicle. ##p < 0.01 versus 27-OHC only. RLU Relative luciferase units