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. Author manuscript; available in PMC: 2017 Jan 15.
Published in final edited form as: Acta Biomater. 2015 Nov 14;30:62–71. doi: 10.1016/j.actbio.2015.11.019

Fig. 4. SPR and ELISPOT analysis.

Fig. 4

SPR measurement of Koff (a, b) and ELISPOT measurements of IFN-γ responses by pESAT-stimulated draining lymph node cells from immunized mice (c), for peptides unheated or heated at 45°C for 7 days. ESAT-Q11 raised antibodies with similar Koff values before and after heating, both as a dry powder and when formulated as nanofibers in buffer (a, representative SPR traces; b, Koff calculated). n=5 (one died from anesthesia in the 45°C dry group), n.s. = p > 0.05 by ANOVA with Tukey post-hoc tests.