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. Author manuscript; available in PMC: 2016 Apr 5.
Published in final edited form as: Clin Cancer Res. 2009 Jun 9;15(13):4374–4381. doi: 10.1158/1078-0432.CCR-09-0334

Fig. 5.

Fig. 5

Adjuvant replication-incompetent UV-treated reovirus also primes antitumor immunity. A, reovirus was UV irradiated in 100 μL volumes in 96-well plates, and titer assessed by an L929 plaque assay. Data are representative of two experiments. B, C, and D, reovirus treated with 480 mJ UV was added to Mel888-preloaded DC (Mel888-adj.UVreo) and assessed in priming assays done as in Fig. 4. Antitumor activity assessed by 51Chromium assay (B) and intracellular IFN-γ production (C) following coculture with Mel888 or irrelevant SKOV-3 cell targets. Points, means of triplicate wells; bars, SE. % shown in C are of CD8+ T cells. D, levels of IL-4, IL-10, and IFN-γ were determined by ELISA after 2 wk of cultures. Columns, means of triplicate wells; bars, SE. Results representative of three independent experiments.