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. Author manuscript; available in PMC: 2016 Apr 5.
Published in final edited form as: Gene Ther. 2008 Apr 10;15(18):1257–1270. doi: 10.1038/gt.2008.58

Figure 1.

Figure 1

Susceptibility of human melanoma cell lines to reovirus-induced cytotoxicity. (a). Mel-888, Mel-624, Mewo and Skmel-28 cells were either left untreated (control) or treated with 10 PFU/cell reovirus. After 48 or 72 h, all cells were harvested and stained with propidium iodide (PI) before fluorescence-activated cell sorting (FACS) acquisition. Cell death was determined by the proportion of cells staining positive for PI. Average data from four to six independent experiments are shown. Error bars represent s.e.m. (b) Photomicrographs of control (top panel) and 10 PFU/cell reovirus-treated cells (lower panel) were taken 48 h after reovirus infection. (c) Melan-A, Skmel-28, Mel-624, Mel-888, Colo829, A375, WM266 and Mewo cells were treated with serial dilutions of reoviral stock solution (6.9 × 108 PFU/ml) and cell survival was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Average data from at least three independent experiments are shown.