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. 2015 Apr 17;1(1):e4. doi: 10.1212/01.NXG.0000464295.65736.da

Figure 3. Quantification of transferrin uptake and cellular localization patterns.

Figure 3

(A) High-content imaging analysis of transferrin fluorophore intensity in positively transfected HeLa cells. All 3 mutations confer nearly a 60% reduction in transferrin uptake compared to wild type (WT). Error bars represent SD between 5 replicate wells. p < 0.05 by Student t test. (B) High-content imaging spot identification revealed a 50% reduction in the number of puncta in cells expressing the G359A mutation. Error bars represent SD among 5 replicate wells. *p < 0.05 by Student t test.