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. 2016 Apr 5;7:427. doi: 10.3389/fmicb.2016.00427

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Copyright © 2016 Potgieter, Nakedi, Ambler, Nel, Garnett, Soares, Mulder and Blackburn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reference protein gene model validation —A0R0A1. A validation of the gene model of the Reference protein A0R0A1 (Glyoxalase/bleomycin resistance protein/dioxygenase). This is an unreviewed predicted entry associated with dioxygenase activity (GO molecular function), of length of 138. Panel (A) illustrates validation of the gene model at the peptide level visualized on the Ensembl genome browser, with a sequence coverage of 99.28% from 13 peptides and a total of 152 MS/MS spectra identified in the Reference proteome database search. Panel (B) shows the identified translation start site (TSS) peptide, illustrating the cleavage of initiator methionine (N-Met) by methionine aminopeptidase (MAP) with Val as the penultimate amino acid. Panel (C) shows a representative MS/MS spectrum for the identified TSS peptide.