Figure 4.

Reference protein frameshift validation —MSHD. Shows the identification of A4ZHT6 (Mycothiol acetyltransferase), a Reference protein inferred from homology. (A) This sequence corresponds to an interrupted coding sequence (ICDS) identified by Deshayes et al. (2007), which they confirmed by resequencing to span a sequencing error in the M. smegmatis mc²155 genome (GenBank accession DQ866865). We present peptide evidence for this protein with eight peptides identified from 50 MS/MS scans after searching the Reference proteome database, as well as identifying a peptide spanning the frameshift position (245) in the corrected sequence—highlighted in red. (B) A representative spectrum of the highlighted peptide, which was identified with four MS/MS scans, supporting the sequence correction at the peptide level.