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. Author manuscript; available in PMC: 2017 Jan 6.
Published in final edited form as: Prog Biophys Mol Biol. 2016 Jan 6;120(1-3):67–76. doi: 10.1016/j.pbiomolbio.2016.01.002

Figure 1.

Figure 1

R25W reduces heteromeric peak tail and steady-state current density in human embryonic kidney (HEK) cells at physiological temperatures (36 ± 1°C). Sample current traces, normalized to cellular capacitance, and recorded from HEK cells coexpressing 1a/1b (A) or 1a/1b-R25W (B). (C) Peak tail current density measured at −50 mV, plotted as a function of pre-pulse potential, and fitted with a Boltzmann function for hERG 1a/1b channels (green) and hERG 1a/1b-R25W channels (blue). (D) Steady-state current measured at the end of 3-second test pulse, normalized to cellular capacitance and plotted as a function of test pulse potential for hERG 1a/1b (green) and hERG 1a/1b-R25W (blue). * indicates statistical significance compared to controls at p < 0.05. n = 6-9.