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. Author manuscript; available in PMC: 2017 Jan 6.
Published in final edited form as: Prog Biophys Mol Biol. 2016 Jan 6;120(1-3):67–76. doi: 10.1016/j.pbiomolbio.2016.01.002

Figure 3.

Figure 3

The hERG 1b-specific mutation R25W reduces hERG 1a and 1b protein levels. (A) Sample western blot from HEK cells transfected with either hERG 1a/1b or hERG 1a/R25W and incubated at either 37°C or 27°C. (B) Total hERG 1a protein relative to actin and normalized to control following 24 hour incubation at 37°C and 27°C. (C) Total hERG 1b protein relative to actin and normalized to control following 24 hour incubation at 37°C and 27°C. Experiments completed on the same day were paired to increase statistical power. (D) Western blot showing hERG protein from cell lysate (Lysate) and co-immunoprecipitation (IP). * indicates statistically significance compared to control at p < 0.05. n = 3-7.