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. Author manuscript; available in PMC: 2017 Jan 6.
Published in final edited form as: Prog Biophys Mol Biol. 2016 Jan 6;120(1-3):67–76. doi: 10.1016/j.pbiomolbio.2016.01.002

Figure 4.

Figure 4

1b-R25W impairs hERG 1b trafficking and surface expression. (A) Western blot depicting reduced mature hERG 1a (155 kDa) and hERG 1b (90 kDa) protein in the presence of 1b-R25W. (B) Mature hERG 1b protein reported as a ratio of total protein for hERG 1a (left) and hERG 1b (right). (C) Western blot displaying lysate (left) and biotinylation assay (right) against hERG display reduced surface hERG in the presence of 1b-R25W. (D) Quantification of biotinylation assay displaying surface hERG 1a (left) and hERG 1b (right) protein levels reported relative to control experiments from the same blot. * indicates statistical significance compared to control at p < 0.05. n = 5-8.