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. Author manuscript; available in PMC: 2017 Jan 6.
Published in final edited form as: Prog Biophys Mol Biol. 2016 Jan 6;120(1-3):67–76. doi: 10.1016/j.pbiomolbio.2016.01.002

Figure 5.

Figure 5

Lysosomal inhibition rescues protein levels in the presence of 1b-R25W. (A) Sample western blot from HEK cells coexpressing hERG 1a with either hERG 1b or 1b-R25W. Cells were incubated for 24 hours in control medium (Untreated) or 1 µM of the lysosomal inhibitor, bafilomycin (Bafilo). (B & C) Total hERG 1a (B) and 1b (C) protein levels relative to actin measured from cells cultured in untreated medium and medium supplemented with 1 µM bafilomycin. Data displayed were acquired from the sample blot displayed in “A”. Overnight treatment with bafilomycin restored total hERG 1a (B) and hERG 1b (C) protein levels to WT levels. (D) Percent increase in total hERG 1a (left) and 1b (right) protein following bafilomycin treatment. Data displayed are from nine experiments completed on three different days. Experiments completed on the same day were paired to increase statistical power. * indicates statistical significance compared to control. n = 3-8