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. Author manuscript; available in PMC: 2017 Jan 6.
Published in final edited form as: Prog Biophys Mol Biol. 2016 Jan 6;120(1-3):67–76. doi: 10.1016/j.pbiomolbio.2016.01.002

Figure 6.

Figure 6

1b-R25W displays a dominant negative phenotype on native IKr. Sample E-4031 sensitive current traces, indicative of IKr, recorded from induced pluripotent stem cell-derive cardiomyocytes (iPSC-CMs) transfected with pcDNA3.1 (Vector, A), WT hERG 1b (B), or 1b-R25W (C). (D) Peak tail IKr, measured at −40 mV, normalized to cellular capacitance, plotted as a function of pre-pulse potential, and fitted with a Boltzmann function for vector (black), WT hERG 1b (green), and 1b-R25W (blue) transfected iPSC-CMs. (E) Steady-state IKr, measured at the end of 3-second test pulse, normalized to cellular capacitance, and plotted as a function of test potential for vector (black), hERG 1b (green), and 1b-R25W (blue) transfected iPSC-CMs. * indicates statistical significance compared to Vector at p < 0.05. n = 6–11.