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. Author manuscript; available in PMC: 2016 Apr 5.
Published in final edited form as: Chem Res Toxicol. 2008 Aug 26;21(10):2051–2060. doi: 10.1021/tx800226m

Figure 2.

Figure 2

Identification of a human Keap1 peptide by LC-MS/MS analysis. Panel A depicts the [M + H]+ and [M + 2H]2+ ions for a carboxyamidomethylated peptide from a Keap1 tryptic digest. The observed monoisotopic mass matches well with the theoretical mass calculated from the sequence (1401.51 Da). Panel B is the product ion spectrum obtained by gas-phase activation of of the [M + 2H]2+ precursor observed above. The peptide sequence with theoretical fragmentation ions are illustrated in the upper left corner of panel B.