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LCMS chromatogram of a tryptic digest of human Keap1. The protein was incubated with 10 mM GSSG, followed by the addition of iodoacetamide and trypsin (see Experimental Procedures). Carboxyamidomethylated cysteine residues are indicated by a solid arrow. Type 1 and type 2 disulfides are indicated by dotted arrows.
