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. Author manuscript; available in PMC: 2016 Apr 5.
Published in final edited form as: J Biol Chem. 2007 Dec 17;283(8):5046–5057. doi: 10.1074/jbc.M709231200

FIGURE 6. S100A1 competes with CaM for binding to intact RyR1 in isolated SR vesicles.

FIGURE 6

A, S100A1 competes RyR1 away from agarose-linked CaM beads at high Ca2+ concentrations, as visualized by a decreased RyR1 band intensity in an anti-RyR Western blot. Each bar graph is the average of at least three images. At the bottom are representative bands from a single blot. Free CaM, a positive control, also competes RyR away from CaM-linked beads in the same protein concentration range. B, S100A1 and CaM compete RyR1 from CaM beads at low (100 nM) Ca2+ concentrations similar to those established for resting skeletal muscle (65).