Fig. 4.

Differential temporal effects of haloperidol (HAL) and olanzapine (OLZ) administration on cell proliferation in hippocampus of adult rat brain. Animals were treated with vehicle, HAL or OLZ, as described in Fig. 4 for 14 and 45 days. All procedures were as described in Wakade et al. (2002). Newly born cells were labeled with bromodeoxyuridine (BrdU) and visualized with DAB immunostaining staining (brown dots), and then stained cells were counted. A, top shows the representative immunohistograms of control-14 day, HAL-14 day, and olanzapine-14 day; bottom shows the control-45 day, HAL-45 day, and olanzapine-45 day. Most of the proliferating cells are in the hilus and subgranular zone of the dentate gyrus. The inset in olanzapine-45 day shows a higher magnification of a group of BrdU-positive cells. B, differential temporal effects on the numbers of proliferating cells (*, p < 0.001 versus vehicle).