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. 2016 Mar 2;6(3):150189. doi: 10.1098/rsob.150189

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 4. — Gad8 Thr6 phosphorylation reduces Gad8-controlled phosphorylation of Tor1 and Tor2. (a) Gad8 phosphorylation of the Gad8-specific substrate Tor1.T1972 in T6A and T6D mutants, along with quantification of relative Tor1.T1972 phosphorylation levels. (b) Gad8 phosphorylation of the Gad8-specific substrate Tor1.T1972 in the S546A mutant. (c) Gad8 phosphorylation of the Gad8-specific substrate Tor2.T1975 in T6A and T6D mutants, along with quantification of relative Tor2.T1975 phosphorylation levels. (d) Gad8-dependent phosphorylation of Rsp6 in T6A and T6D mutants. (e) Gad8 phosphorylation of the Gad8-dependent substrate Psk1 in T6A and T6D mutants.