Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 2;6(3):150189. doi: 10.1098/rsob.150189

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

PMC Copyright notice

Figure 6. — High levels of PKC reduce Gad8 binding to TORC2-Sin1. (a) Gad8.Thr6 resembles a protein kinase C phosphorylation motif that is conserved in other AGC kinases. §Has previously been identified by Powell et al. [37]. (b) Heat stress sensitivity of pck2.Δ and gad8.T6A mutants is rescued by osmotic stabilizer. (c) Pck2 overexpression in T6A and T6D mutants. Pck2 reduces Gad8.S546 phosphorylation through Thr6. (d) Pck2 overexpression reduces Gad8 and Sin1 (TORC2) co-immunoprecipitation. (e) Stress response of gad8 T6A, T6D and S546A mutants following Pck2 overexpression. (f) Measurement of actin remodelling (NETO, new end take off) in T6A and T6D mutants. Hash symbol denotes identical to figure 3c. (g) Schematic suggesting that PKC-dependent regulation of Gad8 Thr6 blocks Gad8 binding to TORC2 and therefore reduces Gad8-dependent TORC2 inhibition and TORC2 activation of Gad8. (c,d) Asterisk indicates a background band.