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. 2016 Mar 16;6(3):150206. doi: 10.1098/rsob.150206

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 4. — Isolated DPB domains of KKT2 and KKT20 can localize at kinetochores. (a) KKT2 DPB has kinetochore localization in G2, metaphase and anaphase. (b,c) W1048A and Y1064A mutations abolish the kinetochore localization of KKT2 DPB (b) but not the full-length protein (c). (d) KKT20 DPB localizes at kinetochores, which is abolished in W99A and Y112A mutants. Note that KKT20 DPB co-localizes with tdTomato-KKT2. (e) The Y112A mutation, but not W99A, abolishes the kinetochore localization of the full-length KKT20 protein. For (a–e), inducible GFP–NLS fusion proteins were expressed with 10 ng ml⁻¹ doxycycline for 1 day. Scale bars, 2 µm.