Table 4.
Summary of peptides of FGF17 identified by lysine-targeted protect and label. Labelled peptides were identified by MALDI-Q-TOF and analysed by MS-digest from the package ProteinProspector v. 5.12.3. A full list of identified peptides is provided in the electronic supplementary material, table S1. The three proteases used for protein digestion were thermolysin (THE), chymotrypsin (CHY) and Glu-C (GLU). The spectrums were shown in the electronic supplementary material, figure S17–S21.
| peptide | sequence | protease | residue | HBS | spectrum | |
|---|---|---|---|---|---|---|
| FGF17 | 1 | GNK(biotin)FAK(biotin)LIVETD | (GLU) | 80–91 | 1 | S17 |
| 2 | GSRVRIK(biotin)GAESEK(biotin)Y | (CHY) | 94–107 | 1 | S18 | |
| 3 | LIGK(biotin)PSGK(biotin)SK(biotin)DCVFTE | (THE) | 116–131 | 1 | S19 | |
| 4 | IK(biotin)RLY | (CHY) | 175–179 | 2 | S18 | |
| 5 | QGQLPFPNHAEK(biotin)QK(biotin)QF | (CHY) | 180–195 | 2 | S18 |