Table 5.
Summary of peptides of FGF20 identified by lysine-targeted protect and label. Labelled peptides were identified by MALDI-Q-TOF and analysed by MS-digest from the package ProteinProspector v. 5.12.3. A full list of identified peptides is provided in the electronic supplementary material, table S1. The two proteases used for protein digestion were thermolysin (THE) and chymotrypsin (CHY). The spectrums were shown in the electronic supplementary material, figure S22–S23.
| peptide | sequence | protease | residue | HBS | spectrum | |
|---|---|---|---|---|---|---|
| FGF20 | 1 | YLGMNDK(biotin)GEL | (CHY) | 118–127 | 1 | S22 |
| 2 | YGSEK(biotin)LTSECIF | (CHY) | 128–139 | 1 | S22 | |
| 3 | K(biotin)HGDTGRRYF | (CHY) | 157–166 | 1 | S22 | |
| 4 | LNK(biotin)DGTPRDGARSK(biotin)RHQK(biotin)FTH | (THE) | 169–189 | 1 | S23 | |
| 5 | K(biotin)DLLMYT | (CHY) | 205–211 | 3 | S22 |