Figure 3. Enrichment efficiency is directly related to probe-target identity.

A set of 10 HCV samples with highest VL was sequenced before and after enrichment with a single-genome, subtype 1a probe set, and for each sample the relative read depth for each probe window was plotted against the maximum identity between target and any probe. Read depth ratio was normalized by giving the most efficiently enriched probe position (in the highly conserved 5’ UTR) a value of 1. Maximal enrichment is observed where probe-target identity exceeds approximately 80% and enrichment decreases dramatically as identity falls below 80%.