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. 2016 Feb 1;6(2):155–164. doi: 10.1002/2211-5463.12032

Figure 3.

Figure 3

YAP1 is identified as a functional target of miR‐497 in hepatocellular carcinoma (HCC). (A) qRT‐PCR and (B) western blot analysis of YAP1 expression in HepG2 cells with miR‐497 or control vectors transfection. n = three independent experiments; *P < 0.05 by t‐test. (C) miR‐497 and its putative binding sequence in the 3′‐UTR of YAP1. The mutant miR‐497 binding site was generated in the complementary site for the seed region of miR‐497 (wt, wild‐type; mt, mutant type). (D) miR‐497 significantly suppressed the luciferase activity that carried wt but not mt 3′‐UTR of YAP1. Anti‐miR‐497 led to a noticeable increase in luciferase activity of wt 3′‐UTR of YAP1. n = three repeats with similar results; *P < 0.05 by t‐test.