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. 2016 Feb 27;6(4):251–263. doi: 10.1002/2211-5463.12027

Figure 5.

Figure 5

S1PR1 3′UTR is targeted by ebv‐miR‐BART16. (A) Schematic representation of the pMIR‐S1PR1 3′UTR reporter construct (upper panel) and prediction of the ebv‐BART16 binding site. The mutated binding site for BART16 is shown in the lower panel. (B) Dual‐luciferase reporter assays for ebv‐miR‐BART16 and S1PR1 3′UTR. Co‐transfection of the S1PR1 WT‐reporter construct with pSG5‐BART16 results in a significant decrease in luciferase activity compared to the empty pMIR reporter vector. Using the S1PR1 reporter with mutated BART16 binding site, no effect was observed. The graph represents the results of at least three independent experiments carried out in duplicate. Error bars show SD. Stars denote: ***P < 0.001.