Table 1.
Primer sequences for PCR analysis
| Genes | Primer sequences (5′–3′) | Length (bp) | T m (°C) |
|---|---|---|---|
| GFP |
F: CAG TGC TTC AGC CGC TAC CC R: TGC CGT TCT TCT GCT TGT CG |
277 | 58 |
| tdTomato |
F: AGG GCG AGG AGG TCA TCA AA R: CAT GGT CTT CTT CTG CAT TAC GG |
416 | 58 |
| SRY |
F: GCT TTC ATT GTG TGG TCT CGT R: CTT GGC GAC TGT GTA TGT GAA G |
309 | 58 |
| GAPDH |
F: GAT GGC CCC TCT GGG AAA CTG TG R: GGA CGC CTG CTT CAC CAC CTT CT |
404 | 58 |
| EF1α outside |
F: GCG TTT TAG CGT ATA TGT TCG GCG A R: TCA CGA CAC CTA AAA TAA AAA AAA |
558 | 53 |
| EF1α inside |
F: TTG TTG TAG GGA GTT TAA AAT GGA G R: CCA CCC ACT CAA TAT AAA AAA ACT C |
231 | 52 |
| CMV outside |
F: GTT TGG TTG ATC GTT TAA CGA TTT TC R: AAC GAT TCA CTA AAC CAA CTC TAC TT |
497 | 53 |
| CMV inside |
F: TGA TTT TAT GGG ATT TTT TTA TTT G R: ATT CAC TAA ACC AAC TCT ACT TAT ATA AAC |
278 | 52 |