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. 2016 Mar 14;6(4):358–368. doi: 10.1002/2211-5463.12047

Figure 3.

Figure 3

(I) Retention of intron‐3 visualized by RT‐PCR during pre‐mRNA processing of the asn‐2 gene in N. crassa. Strains Δmak‐2 mutant and St.L.74A were incubated for 5 h and 16 h in high‐ (10 mm) (↑) or low‐Pi (100 μm) (↓) at pH 5.4 and pH 8.0 in the absence of antifungal (control) (A, B), with amphotericin B (C, D), and with ketoconazole (E, F). (M) Molecular weight ladder. Sizes expected for the amplified fragments were 49 bp and 188 bp for nonretention or retention of the intron, respectively. (II) Schematic overview of intron‐3 retention, as compared to the genomic DNA and mRNA organization of the asn‐2 gene of N. crassa.