Figure 7.

(I) Retention of intron‐1 visualized by RT‐PCR during pre‐mRNA processing of the fluffy gene in N. crassa. Strains Δmak‐2 mutant and St.L.74A were incubated for 5 h and 16 h in high‐ (10 mm) (↑) or low‐Pi (100 μm) (↓) at pH 8.0 in the absence (A) and presence (B) of ketoconazole. (M) Molecular weight ladder. Sizes expected for the amplified fragments are 149 and 299 bp for nonretention or retention of the intron, respectively. (II) Schematic overview of intron‐1 retention, as compared to the genomic DNA and mRNA organization of the fluffy gene of N. crassa.