Table 1.

Primers used in RT‐PCR and/or qRT‐PCR experiments

Gene product name (gene name)	IDa	Primer sequences (5′‐3′)	Amplicon length (bp)	Amplicon spliced (bp)	Intron analyzed	Experiments
Asparagine synthetase 2 (asn‐2)	NCU04303
	F:	ATCCGTCACCGTGGTCCTGAT	188	49	Intron 3	RT‐PCR
	R:	TGTTGTGGCAAGTGACGCTGC
	F:	CAACCTTTCGCCCAACC	63	–	Intron 3	qRT‐PCR
	R:	TTGACTCGATTGACACTTTTAT
	F:	GCGGCAGCGTCACTTG	142	57	Intron 4	RT‐PCR
	R:	CCGACGATACTGAGACGCT
	F:	TGCGGATAAGACGATGGTTG	182	112	Intron 5	RT‐PCR
	R:	TGCCAAGCGTCTTGTTTCTA
C6‐zinc finger (fluffy)	NCU08726
	F:	GCCAAGACAACACCTAACACCGAA	299	149	Intron 1	RT‐PCR
	R:	AGATGGTCCTTGGTACGCCATTTC
CaaX farnesyltransferase beta subunit Ram1 (ram‐1)	NCU05999
	F:	CAACCCAACAACGAGGCCCATGGC	503	313	Intron 3	RT‐PCR
	R:	CGACTATAGAGACTCTCCGGCGCA
Actin	NCU04173
	F:	GTATGTGCAAGGCCGGTTTCG	305	109	Introns 3/4	RT‐PCR qRT‐PCR
	R:	TCCTTCTGGCCCATACCGATCATG
Tubulin alpha‐1	NCU09132
	F:	CCTCGTCTTCCACTCCTTCGG	445	190	Intron 4	RT‐PCR qRT‐PCR
	R:	ACTGTGTTCGAGGGTGCTGTG

^aGene accession number at the N. crassa genome database at the Broad Institute.