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. Author manuscript; available in PMC: 2016 Apr 5.
Published in final edited form as: J Immunol. 2014 Jul 25;193(5):2118–2126. doi: 10.4049/jimmunol.1400056

Figure 6. Ability of 21-OH specific T cells to lyse target cells expressing full-length 21-OH protein.

Figure 6

HLA-A2 restricted 21-OH342-350 specific T cell clone was co-incubated overnight at indicated E:T ratios with B cells either transduced with lentiviral vector encoding the full-length 21-OH protein and GFP or with CMTMR labeled untransduced B cells. Panel A shows FACS staining. Top two panels show target cells in the absence of the 21-OH342-350 specific T cell clone. Bottom four panels show co-cultures between T cell clone and target cells at the indicated E/T ratios. Increasing numbers of T cells are shown in the bottom left corner of each dot plot. The percentage of GFP+ and CMTMR+ labeled cells is shown in each dot plot. Panel B shows percentage of specific lysis of 21-OH positive target cells.