Figure 2.
Effects of miR-93 on chemo-sensitivity of bladder cancer cells in vitro and in vivo.
Notes: (A) T24 and (B) RT4 cells (1×105) were seeded into six-well plates and subjected to transfection with an miR-93 inhibitor the next day. Twenty-four hours after transfection, the plates were co-cultured with cisplatin with doses of 0, 1.25, 2.5, 5, and 10 μM given in a single fraction. After 8 days, the cells were fixed with 10% paraformaldehyde and stained with 1% crystal violet in 70% ethanol. Cell viability under the treatment of cisplatin was shown as fold change of cell viability normalized to that of the cells treated with saline. Each bar represents the mean ± standard deviation. The results shown were repeated in three independent experiments. (C) and (D) T24 cells (5×106/mouse) stably transfected with miR-93 antagomir (Anta) or control antagomir were injected into the right thigh of 5-week-old BALB/C nude mice. When tumors reached 8 mm in diameter, the mice bearing either miR-93-repressing tumors or control antagomir tumors were randomly divided into two groups. One group received cisplatin treatment (5 μM), and the other group served as controls. Tumor sizes were measured twice a week. There was no difference in tumor weights and volumes between the group transfected with miR-93 antagomir and the group transfected with control antagomir, but after receiving chemotherapy, the tumor weights and volumes of the stably repressing miR-93 group were significantly lower than those of control antagomir group. Each bar represents the mean ± standard deviation. P-values: Student’s t-test.
Abbreviation: NC, negative control.