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. 2016 Mar 29;9:1813–1822. doi: 10.2147/OTT.S97399

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© 2016 Liu et al. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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MiR-93 inhibitor enhanced cisplatin-induced cell death by regulation of DNA damage-induced apoptosis.

Notes: (A) miR-93 had no effect on bladder cancer cell growth. (B) Repressing miR-93 did not change the activity of CASP3, but after receiving chemotherapy, activity was detected in T24 and RT4 cells transfected with miR-93 inhibitor. (C) Western blotting was used to determine the expression of cleaved CASP3 in T24 or RT4 cells transfected with miR-93 inhibitor. (D) The T24 and RT4 cells transfected with miR-93 inhibitor for 24 hours followed by exposure to 5 μM of cisplatin for additional 24 hours, and analyzed by γH2AX foci staining. (E) Quantification of γH2AX foci-positive cells of T24 and RT4 cells transfected with miR-93 inhibitor. The cells with γH2AX foci >5 were counted as positive cells and expressed as a percentage. Results are representative of at least three independent experiments and shown as the mean ± standard deviation. P-values: Student’s t-test.

Abbreviations: Anta, antagomir; NC, negative control; DAPI, 4′,6-diamidino-2-phenylindole; OD, optical density.

MiR-93 inhibitor enhanced cisplatin-induced cell death by regulation of DNA damage-induced apoptosis.

Notes: (A) miR-93 had no effect on bladder cancer cell growth. (B) Repressing miR-93 did not change the activity of CASP3, but after receiving chemotherapy, activity was detected in T24 and RT4 cells transfected with miR-93 inhibitor. (C) Western blotting was used to determine the expression of cleaved CASP3 in T24 or RT4 cells transfected with miR-93 inhibitor. (D) The T24 and RT4 cells transfected with miR-93 inhibitor for 24 hours followed by exposure to 5 μM of cisplatin for additional 24 hours, and analyzed by γH2AX foci staining. (E) Quantification of γH2AX foci-positive cells of T24 and RT4 cells transfected with miR-93 inhibitor. The cells with γH2AX foci >5 were counted as positive cells and expressed as a percentage. Results are representative of at least three independent experiments and shown as the mean ± standard deviation. P-values: Student’s t-test.

Abbreviations: Anta, antagomir; NC, negative control; DAPI, 4′,6-diamidino-2-phenylindole; OD, optical density.