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. 2016 Mar 29;9:1801–1812. doi: 10.2147/OTT.S96535

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© 2016 Zhang et al. This work is published and licensed by Dove Medical Press Limited

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Effect of Twist2 on kidney cancer cell migration and invasion.

Notes: (A, B) The migration of 786-0 and ACHN cells was performed in in vitro scratch wound healing assay, and photographs were taken 0, 24, and 48 hours after the wound was made. (C, D) The invasion of 786-0 and ACHN cells was performed by Transwell assay, and photographs were taken at 48 hours after incubation in Matrigel precoated Transwell chamber (× 200). *P<0.001 compared with shNC or NC.

Abbreviations: shNC, recombinant lentivirus pLKO.1-EGFP-scramble shRNA; NC, black pWPXL; shTwist2, recombinant lentivirus pLKO.1-EGFP-Twist2.