Figure 5.
(a) Negative ion ATD profile (unsmoothed) of Man6GlcNAc2 ([M+H2PO4]− ion) from gp120. (b) Single ion plots of the diagnostic 6-antenna-specific fragment ions from Man6GlcNAc2 obtained from gp120 (smoothing algorithm, mean 2×2). (c) Negative ion CID spectrum ([M+H2PO4]− ion) of Man6GlcNAc2 (d1 isomer, 14, from bovine thyroglobulin). (d) Negative ion CID spectrum ([M+H2PO4]− ion) of Man6GlcNAc2 (d2 isomer, 12, from gp120 (JRCSF)). (e) Negative ion CID spectrum ([M+H2PO4]− ion) of Man6GlcNAc2 (d3 isomer, 13), extracted from the right-hand portion of the ATD peak shown in 5a and 5b.