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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Mass Spectrom. 2016 Mar;51(3):219–235. doi: 10.1002/jms.3738

Figure 7.

Figure 7

(a) Negative ion ATD profile (smoothing algorithm, mean 2×2) of Man GlcNAc ([M+H PO ]− ion) from gp120. (b) Single ion plots of the diagnostic 6-antenna-specific fragment ions from Man8GlcNAc2 (mainly isomer 7) obtained from gp120 (smoothing algorithm, mean 2×2). (c) Negative ion ATD profiles (smoothing algorithm, mean 2×2) of the d1d1d2 (5) and d1d1d3 (7) isomers of Man8GlcNAc2 ([M+H2PO4]− ions). Spectra were from different samples and normalized to 100%. (d) Negative ion CID spectrum ([M+H2PO4]− ion) of Man8GlcNAc2 (d1d1d3 isomer, 7, from gp120). (e) Negative ion CID spectrum ([M+H2PO4] ion) of Man8GlcNAc2 (d1d1d2 isomer, 5, from a Saccharomyces cerevisiae mutanty117. (f) Negative ion CID spectrum ([M+H2PO4]− ion) of Man8GlcNAc2 (d1d2d3 isomer, 6, from gp120 expressed in CHO cells in the presence of the α-mannosidase inhibitor NB-DNJ.