. Author manuscript; available in PMC: 2017 Mar 1.

Published in final edited form as: J Mass Spectrom. 2016 Mar;51(3):219–235. doi: 10.1002/jms.3738

Table 3.

, Fragment ions in the negative ion CID spectra that uniquely define the isomers of the high-mannose N-glycans reported in this paper

Glycan	Isomer	No.	m/z ([M+H₂PO₄]⁻)	Structural evidence
Glycan	Isomer	No.	m/z ([M+H₂PO₄]⁻)	D	D-18	^0,4 A	^0,3 A	D’	D’-18
Man4	-	18	1169.3	485.2	467.2	413.1	395.1	-	-
Man5	-	17	1331.4	647.2	629.2	575.2	545.2	323.1	305.1
Man6	d1	14	1493.5	647.2	629.2	575.2	545.2	323.1	305.1
	d2	12		809.3	791.3	737.2	707.2	323.1	305.1
	d3	13		809.3	791.3	737.2	707.2	485.2	467.2
Man7	d1d1	11	1655.5	647.2	629.2	575.2	545.2	323.1	305.1
	d1d2	8		809.3	791.3	737.2	707.2	323.1	305.1
	d1d3	10		809.3	791.3	737.2	707.2	485.2	467.2
	d2d3	9		971.3	953.3	899.3	869.3	485.2	467.2
Man8	d1d1d2	5	1817.6	809.3	791.3	737.2	707.2	-	-
	d1d1d3	7		809.3	791.3	737.2	707.2	485.2	467.2
	d1d2d3	6		971.3	953.3	899.2	869.3	485.2	467.2
Man9	d1d1d2d3	4	1979.6	971.3	953.3	899.3	869.3	485.2	467.2
Man9		26	1979.6	809.3	791.3	737.2	707.2	485.2	467.2
Man10	-	27	2141.7	971.3	9533	899.3	869.3	485.2	467.2