Fig 4. HUNK knockdown inhibits JIMT-1 xenograft mammary tumor growth.

A) Time to tumor size of 600 mm³ was significantly longer for mice with HUNKshRNA expressing tumors relative to control shRNA derived tumors based on Kaplan–meier survival curve estimates; p < 0.0001, log-rank test. B) Immunoblot analysis of control and HUNK shRNA expressing JIMT-1 cell lysates for phospho-JNK and total JNK expression. C) Immunoblot analysis of control and HUNK shRNA expressing JIMT-1 cell lysates for phospho-EGFR and total EGFR expression. D) Immunoblot analysis of control and HUNK shRNA expressing JIMT-1 cell lysates for phospho-AKT and total AKT expression E) Control and HUNK shRNA expressing JIMT-1 cells were treated with inhibitors targeting AKT, PLC, JNK, PI3K, MEK, and EGFR for 24 hrs and evaluated for Caspase-3 activity as a measure of apoptosis. p-values were determined by student’s T-test.