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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Methods Enzymol. 2015 Sep 26;569:3–22. doi: 10.1016/bs.mie.2015.08.008

Figure 1. Application of BioID method to LaA.

Figure 1

The N-terminus of a lamin can be fused to a promiscuous biotin-ligase, which prematurely releases BioAMP. Cells stably expressing the fusion protein in biotin containing media leads to the selective biotinylation of proteins within proximity of the BioID-lamin in living cells. After cell lysis and solubilization, biotinylated proteins are affinity purified using streptavidin-conjugated beads, allowing for stringent wash conditions. Candidate proteins are subsequently analyzed by IB analysis and identified by MS.